AFG3 | GeneID:856737 | Saccharomyces cerevisiae

Gene Summary

[ - ] NCBI Entrez Gene

Gene ID 856737 Official Symbol AFG3
Locus YER017C Gene Type protein-coding
Synonyms YTA10
Full Name N/A
Description Afg3p
Chromosome N/A
Also Known As Component, with Yta12p, of the mitochondrial inner membrane m-AAA protease that mediates degradation of misfolded or unassembled proteins and is also required for correct assembly of mitochondrial enzyme complexes
Summary N/A

Orthologs and Paralogs

[ - ] Homologs - NCBI's HomoloGene Group: 24990

ID Symbol Protein Species
GeneID:114896 Afg3l1 NP_473411.2 Mus musculus
GeneID:361436 Afg3l1 XP_341715.2 Rattus norvegicus
GeneID:532875 LOC532875 XP_612083.3 Bos taurus
GeneID:570430 LOC570430 XP_001920635.1 Danio rerio
GeneID:856737 AFG3 NP_010933.1 Saccharomyces cerevisiae
GeneID:4618406 AGOS_AAR025C NP_982566.1 Eremothecium gossypii

Gene Classification

[ - ] Gene Ontology

IDCategoryGO Term
GO:0016021 Component integral to membrane
GO:0005745 Component m-AAA complex
GO:0016020 Component membrane
GO:0005743 Component mitochondrial inner membrane
GO:0031966 Component mitochondrial membrane
GO:0005739 Component mitochondrion
GO:0016887 Function ATPase activity
GO:0005524 Function ATP binding
GO:0016787 Function hydrolase activity
GO:0046872 Function metal ion binding
GO:0004222 Function metalloendopeptidase activity
GO:0008237 Function metallopeptidase activity
GO:0017111 Function nucleoside-triphosphatase activity
GO:0000166 Function nucleotide binding
GO:0008233 Function peptidase activity
GO:0008270 Function zinc ion binding
GO:0030163 Process protein catabolic process
GO:0006461 Process protein complex assembly
GO:0045041 Process protein import into mitochondrial intermembrane space
GO:0006508 Process proteolysis
GO:0006465 Process signal peptide processing

Protein Sequences

[ - ] Protein Accession Number Cloud [ GenPept ]

AAB64550   CAA56953   NP_010933   P39925   S46611  

Gene Interactions

[ - ] BioGRID Gene Product Interaction Database

Symbol Interaction Binary Experiment Source
ARD1 ARD1 / AFG3 Synthetic Growth Defect Pan X (2006)
BRE1 BRE1 / AFG3 Synthetic Growth Defect Pan X (2006)
ENP1 AFG3 / ENP1 Affinity Capture-MS Ho Y (2002)
ILV1 ILV1 / AFG3 Phenotypic Suppression Chen X (2005)
LGE1 LGE1 / AFG3 Synthetic Growth Defect Pan X (2006)
LPD1 AFG3 / LPD1 Affinity Capture-MS Ho Y (2002)
MBA1 AFG3 / MBA1 Dosage Rescue Rep M (1996)
NAT1 NAT1 / AFG3 Synthetic Growth Defect Pan X (2006)
OMA1 AFG3 / OMA1 Phenotypic Enhancement Kaser M (2003)
OXA1 AFG3 / OXA1 Dosage Rescue Rep M (1996)
PHB1 AFG3 / PHB1 Affinity Capture-MS Gavin AC (2006)
PHB1 PHB1 / AFG3 Affinity Capture-Western Tatsuta T (2005)
PHB2 AFG3 / PHB2 Affinity Capture-MS Gavin AC (2006)
PIM1 AFG3 / PIM1 Dosage Rescue Rep M (1996)
RPD3 RPD3 / AFG3 Synthetic Growth Defect Lin YY (2008)
YME1 YME1 / AFG3 Synthetic Lethality Lemaire C (2000)
YTA12 AFG3 / YTA12 Affinity Capture-MS Collins SR (2007)
YTA12 AFG3 / YTA12 Affinity Capture-MS Gavin AC (2006)
YTA12 YTA12 / AFG3 Affinity Capture-MS Gavin AC (2006)
YTA12 AFG3 / YTA12 Affinity Capture-Western Arlt H (1996)
YTA12 YTA12 / AFG3 Affinity Capture-Western Arlt H (1996)
YTA12 AFG3 / YTA12 Affinity Capture-Western Arlt H (1998)
YTA12 YTA12 / AFG3 Affinity Capture-Western Arlt H (1998)
YTA12 AFG3 / YTA12 Co-fractionation Nolden M (2005)
YTA12 AFG3 / YTA12 Phenotypic Enhancement Arlt H (1998)
YTA12 AFG3 / YTA12 Phenotypic Enhancement Rep M (1996)
YTA12 AFG3 / YTA12 Synthetic Growth Defect Nolden M (2005)

Selected Publications

[ - ] Gene-related publications indexed at PubMed

  1. [ + ] Collins SR, et al. (2007) "Toward a comprehensive atlas of the physical interactome of Saccharomyces cerevisiae." Mol Cell Proteomics. 6(3):439-450. PMID:17200106
  2. [ + ] Reinders J, et al. (2006) "Toward the complete yeast mitochondrial proteome: multidimensional separation techniques for mitochondrial proteomics." J Proteome Res. 5(7):1543-1554. PMID:16823961
  3. [ + ] Pan X, et al. (2006) "A DNA integrity network in the yeast Saccharomyces cerevisiae." Cell. 124(5):1069-1081. PMID:16487579
  4. [ + ] Gavin AC, et al. (2006) "Proteome survey reveals modularity of the yeast cell machinery." Nature. 440(7084):631-636. PMID:16429126
  5. [ + ] Nolden M, et al. (2005) "The m-AAA protease defective in hereditary spastic paraplegia controls ribosome assembly in mitochondria." Cell. 123(2):277-289. PMID:16239145
  6. [ + ] Chen X, et al. (2005) "Enhanced mitochondrial degradation of yeast cytochrome c with amphipathic structures." Curr Genet. 47(2):67-83. PMID:15605252
  7. [ + ] Tatsuta T, et al. (2005) "Formation of membrane-bound ring complexes by prohibitins in mitochondria." Mol Biol Cell. 16(1):248-259. PMID:15525670
  8. [ + ] Korbel D, et al. (2004) "Membrane protein turnover by the m-AAA protease in mitochondria depends on the transmembrane domains of its subunits." EMBO Rep. 5(7):698-703. PMID:15205678
  9. [ + ] Kaser M, et al. (2003) "Oma1, a novel membrane-bound metallopeptidase in mitochondria with activities overlapping with the m-AAA protease." J Biol Chem. 278(47):46414-46423. PMID:12963738
  10. [ + ] Esser K, et al. (2002) "A novel two-step mechanism for removal of a mitochondrial signal sequence involves the mAAA complex and the putative rhomboid protease Pcp1." J Mol Biol. 323(5):835-843. PMID:12417197
  11. [ + ] Ho Y, et al. (2002) "Systematic identification of protein complexes in Saccharomyces cerevisiae by mass spectrometry." Nature. 415(6868):180-183. PMID:11805837
  12. [ + ] Lemaire C, et al. (2000) "Absence of the mitochondrial AAA protease Yme1p restores F0-ATPase subunit accumulation in an oxa1 deletion mutant of Saccharomyces cerevisiae." J Biol Chem. 275(31):23471-23475. PMID:10816574
  13. [ + ] Arlt H, et al. (1998) "The formation of respiratory chain complexes in mitochondria is under the proteolytic control of the m-AAA protease." EMBO J. 17(16):4837-4847. PMID:9707443
  14. [ + ] Dietrich FS, et al. (1997) "The nucleotide sequence of Saccharomyces cerevisiae chromosome V." Nature. 387(6632 Suppl):78-81. PMID:9169868
  15. [ + ] Arlt H, et al. (1996) "The YTA10-12 complex, an AAA protease with chaperone-like activity in the inner membrane of mitochondria." Cell. 85(6):875-885. PMID:8681382
  16. [ + ] Goffeau A, et al. (1996) "Life with 6000 genes." Science. 274(5287):546, 563-546, 567. PMID:8849441
  17. [ + ] Rep M, et al. (1996) "Promotion of mitochondrial membrane complex assembly by a proteolytically inactive yeast Lon." Science. 274(5284):103-106. PMID:8810243
  18. [ + ] Rep M, et al. (1996) "Three genes for mitochondrial proteins suppress null-mutations in both Afg3 and Rca1 when over-expressed." Curr Genet. 30(3):206-211. PMID:8753648
  19. [ + ] Tauer R, et al. (1994) "Yta10p, a member of a novel ATPase family in yeast, is essential for mitochondrial function." FEBS Lett. 353(2):197-200. PMID:7926051
  20. [ + ] Guelin E, et al. (1994) "Sequence of the AFG3 gene encoding a new member of the FtsH/Yme1/Tma subfamily of the AAA-protein family." Yeast. 10(10):1389-1394. PMID:7900428