AFR1 | GeneID:851658 | Saccharomyces cerevisiae

Gene Summary

[ - ] NCBI Entrez Gene

Gene ID 851658 Official Symbol AFR1
Locus YDR085C Gene Type protein-coding
Synonyms
Full Name N/A
Description Afr1p
Chromosome N/A
Also Known As Alpha-factor pheromone receptor regulator, negatively regulates pheromone receptor signaling; required for normal mating projection (shmoo) formation; required for Spa2p to recruit Mpk1p to shmoo tip during mating; interacts with Cdc12p
Summary N/A

Orthologs and Paralogs

[ - ] Homologs - NCBI's HomoloGene Group: 48620

ID Symbol Protein Species
GeneID:851658 AFR1 NP_010370.1 Saccharomyces cerevisiae
GeneID:2892515 KLLA0C11891g XP_452731.1 Kluyveromyces lactis
GeneID:4623122 AGOS_AGR154C NP_986820.1 Eremothecium gossypii

Gene Classification

[ - ] Gene Ontology

IDCategoryGO Term
GO:0001400 Component mating projection base
GO:0005057 Function receptor signaling protein activity
GO:0000767 Process cell morphogenesis during conjugation
GO:0000753 Process cellular morphogenesis during conjugation with cellular fusion
GO:0034605 Process cellular response to heat
GO:0000750 Process pheromone-dependent signal transduction involved in conjugation with cellular fusion
GO:0008277 Process regulation of G-protein coupled receptor protein signaling pathway

Protein Sequences

[ - ] Protein Accession Number Cloud [ GenPept ]

Gene Interactions

[ - ] BioGRID Gene Product Interaction Database

Symbol Interaction Binary Experiment Source
BOI1 BOI1 / AFR1 Two-hybrid Tong AH (2002)
CDC12 AFR1 / CDC12 Affinity Capture-Western Giot L (1997)
CDC12 CDC12 / AFR1 Two-hybrid DeMattei CR (2000)
CDC12 AFR1 / CDC12 Two-hybrid Konopka JB (1995)
GCN4 GCN4 / AFR1 Synthetic Rescue Tavernarakis N (1995)
GCN5 AFR1 / GCN5 Synthetic Lethality Tavernarakis N (1995)
IQG1 IQG1 / AFR1 Two-hybrid Osman MA (1998)
SEN15 SEN15 / AFR1 Affinity Capture-MS Ho Y (2002)
STE2 STE2 / AFR1 Dosage Rescue Davis C (1998)
YRA1 YRA1 / AFR1 Affinity Capture-MS Kashyap AK (2005)

Selected Publications

[ - ] Gene-related publications indexed at PubMed

  1. [ + ] Changwei Z, et al. (2007) "Afr1p has a role in regulating the localization of Mpk1p at the shmoo tip in Saccharomyces cerevisiae." FEBS Lett. 581(14):2670-2674. PMID:17509572
  2. [ + ] Kashyap AK, et al. (2005) "Biochemical and genetic characterization of Yra1p in budding yeast." Yeast. 22(1):43-56. PMID:15584090
  3. [ + ] Ho Y, et al. (2002) "Systematic identification of protein complexes in Saccharomyces cerevisiae by mass spectrometry." Nature. 415(6868):180-183. PMID:11805837
  4. [ + ] Tong AH, et al. (2002) "A combined experimental and computational strategy to define protein interaction networks for peptide recognition modules." Science. 295(5553):321-324. PMID:11743162
  5. [ + ] DeMattei CR, et al. (2000) "Point mutations identify a conserved region of the saccharomyces cerevisiae AFR1 gene that is essential for both the pheromone signaling and morphogenesis functions." Genetics. 155(1):43-55. PMID:10790383
  6. [ + ] Davis C, et al. (1998) "Afr1p regulates the Saccharomyces cerevisiae alpha-factor receptor by a mechanism that is distinct from receptor phosphorylation and endocytosis." Genetics. 148(2):625-635. PMID:9504911
  7. [ + ] Osman MA, et al. (1998) "Iqg1p, a yeast homologue of the mammalian IQGAPs, mediates cdc42p effects on the actin cytoskeleton." J Cell Biol. 142(2):443-455. PMID:9679143
  8. [ + ] Giot L, et al. (1997) "Functional analysis of the interaction between Afr1p and the Cdc12p septin, two proteins involved in pheromone-induced morphogenesis." Mol Biol Cell. 8(6):987-998. PMID:9201710
  9. [ + ] Jacq C, et al. (1997) "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV." Nature. 387(6632 Suppl):75-78. PMID:9169867
  10. [ + ] Goffeau A, et al. (1996) "Life with 6000 genes." Science. 274(5287):546, 563-546, 567. PMID:8849441
  11. [ + ] Konopka JB, et al. (1995) "AFR1 promotes polarized apical morphogenesis in Saccharomyces cerevisiae." Mol Cell Biol. 15(2):723-730. PMID:7823940
  12. [ + ] Tavernarakis N, et al. (1995) "Transcriptional interference caused by GCN4 overexpression reveals multiple interactions mediating transcriptional activation." Mol Gen Genet. 247(5):571-578. PMID:7603436
  13. [ + ] Konopka JB, et al. (1993) "AFR1 acts in conjunction with the alpha-factor receptor to promote morphogenesis and adaptation." Mol Cell Biol. 13(11):6876-6888. PMID:8413281