AAK1 | GeneID:22848 | Homo sapiens

Gene Summary

[ - ] NCBI Entrez Gene

Gene ID 22848 Official Symbol AAK1
Locus N/A Gene Type protein-coding
Synonyms DKFZp686F03202; DKFZp686K16132; FLJ23712; FLJ25931; FLJ31060; FLJ42882; FLJ45252; KIAA1048; MGC138170; MGC164568; MGC164570
Full Name AP2 associated kinase 1
Description AP2 associated kinase 1
Chromosome 2p14
Also Known As adaptor-associated kinase 1
Summary Adaptor-related protein complex 2 (AP-2 complexes) functions during receptor-mediated endocytosis to trigger clathrin assembly, interact with membrane-bound receptors, and recruit encodytic accessory factors. This gene encodes a member of the SNF1 subfamily of Ser/Thr protein kinases. The protein interacts with and phosphorylates a subunit of the AP-2 complex, which promotes binding of AP-2 to sorting signals found in membrane-bound receptors and subsequent receptor endocytosis. Its kinase activity is stimulated by clathrin. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq]

Orthologs and Paralogs

[ - ] Homologs - NCBI's HomoloGene Group: 75032

ID Symbol Protein Species
GeneID:22848 AAK1 NP_055726.3 Homo sapiens
GeneID:269774 Aak1 NP_001035195.1 Mus musculus
GeneID:419512 AAK1 XP_417663.2 Gallus gallus
GeneID:474625 AAK1 XP_531855.2 Canis lupus familiaris
GeneID:500244 Aak1 XP_575594.2 Rattus norvegicus
GeneID:532546 AAK1 XP_611658.3 Bos taurus
GeneID:736127 AAK1 XP_001138098.1 Pan troglodytes
GeneID:817846 AT2G32850 NP_565756.1 Arabidopsis thaliana
GeneID:4346601 Os09g0279100 NP_001062756.1 Oryza sativa

Antibodies

[ - ] Monoclonal and Polyclonal Antibodies

No. Provider Product No. Description
1 abcam ab66005 AAK1 antibody (ab66005); Rabbit polyclonal to AAK1
2 abcam ab59740 AAK1 antibody (ab59740); Rabbit polyclonal to AAK1
3 abgent AP7861b AAK1 Antibody (C-term); Purified Rabbit Polyclonal Antibody (Pab)
4 abnova H00022848-M02 AAK1 monoclonal antibody (M02), clone 4B10; Mouse monoclonal antibody raised against a partial recombinant AAK1.
5 acris AP14706PU-N AAK1 (C-term); antibody Ab
6 acris AP17859PU-N AAK1 N-term; antibody Ab
7 scbt AAK1 AAK1 Antibody / AAK1 Antibodies;
8 sigma HPA017931 Anti-AAK1 antibody produced in rabbit ;

Exon, Intron and UTRs

Exon, Intron and UTRs of AAK1 Gene Transcript Isoforms

CpG near TSS

CpG dinucleotides near Transcription Start Site of AAK1 Gene

Gene Classification

[ - ] Gene Ontology

IDCategoryGO Term
GO:0005905 Component coated pit
GO:0005886 Component plasma membrane
GO:0005524 Function ATP binding
GO:0000166 Function nucleotide binding
GO:0004674 Function protein serine/threonine kinase activity
GO:0016740 Function transferase activity
GO:0006468 Process protein amino acid phosphorylation

RefSeq Isoforms

[ - ] RefSeq Annotation and UniProt Database

No. RefSeq RNA RefSeq Protein UniProt Equivalent
1 NM_014911  UCSC Browser NP_055726

Transcript Sequences

[ - ] Transcript Accession Number Cloud [ GenBank ]

Protein Sequences

[ - ] Protein Accession Number Cloud [ GenPept ]

Mutations and SNPs

[ - ] NCBI's dbSNP

Chemicals and Drugs

[ - ] Comparative Toxicogenomics Database from MDI Biological Lab

Curated [chemical–gene interactions|chemical–disease|gene–disease] data were retrieved from the Comparative Toxicogenomics Database (CTD), Mount Desert Island Biological Laboratory, Salisbury Cove, Maine. World Wide Web (URL: http://ctd.mdibl.org/). [Jan. 2009].
Chemical and Interaction
Acetaminophen
  • Acetaminophen affects the expression of AAK1 mRNA
17562736

Gene and Diseases

[ - ] Gene and Diseases [Data source: CTD]

Curated [chemical–gene interactions|chemical–disease|gene–disease] data were retrieved from the Comparative Toxicogenomics Database (CTD), Mount Desert Island Biological Laboratory, Salisbury Cove, Maine. World Wide Web (URL: http://ctd.mdibl.org/). [Jan. 2009].
Disease Name Relationship PubMed
Hepatitis, Toxic inferred via Acetaminophen 2444490, 16081117, 16177239, 15968718, 16227642, 14986274, 17562736, 17522070
Hyperalgesia inferred via Acetaminophen 16870215
Liver Failure, Acute inferred via Acetaminophen 16871587, 17185352
Pain inferred via Acetaminophen 16870215

Transcript Cluster

[ - ] NCBI's UniGene

Selected Publications

[ - ] Gene-related publications indexed at PubMed

  1. [ + ] Henderson DM, et al. (2007) "A novel AAK1 splice variant functions at multiple steps of the endocytic pathway." Mol Biol Cell. 18(7):2698-2706. PMID:17494869
  2. [ + ] Beausoleil SA, et al. (2006) "A probability-based approach for high-throughput protein phosphorylation analysis and site localization." Nat Biotechnol. 24(10):1285-1292. PMID:16964243
  3. [ + ] Schmid EM, et al. (2006) "Role of the AP2 beta-appendage hub in recruiting partners for clathrin-coated vesicle assembly." PLoS Biol. 4(9):e262. PMID:16903783
  4. [ + ] Collawn JF, et al. (2006) "Unlocking the mysteries of Na+-K+-ATPase endocytosis: phosphorylation is the key." Am J Respir Cell Mol Biol. 35(1):1-2. PMID:16782842
  5. [ + ] Kimura K, et al. (2006) "Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes." Genome Res. 16(1):55-65. PMID:16344560
  6. [ + ] Takahashi T, et al. (2006) "Endocytic Ark/Prk kinases play a critical role in adriamycin resistance in both yeast and mammalian cells." Cancer Res. 66(24):11932-11937. PMID:17178891
  7. [ + ] Hillier LW, et al. (2005) "Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Nature. 434(7034):724-731. PMID:15815621
  8. [ + ] Kim JE, et al. (2005) "Global phosphoproteome of HT-29 human colon adenocarcinoma cells." J Proteome Res. 4(4):1339-1346. PMID:16083285
  9. [ + ] Beausoleil SA, et al. (2004) "Large-scale characterization of HeLa cell nuclear phosphoproteins." Proc Natl Acad Sci U S A. 101(33):12130-12135. PMID:15302935
  10. [ + ] Ota T, et al. (2004) "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat Genet. 36(1):40-45. PMID:14702039
  11. [ + ] Mishra SK, et al. (2004) "Dual engagement regulation of protein interactions with the AP-2 adaptor alpha appendage." J Biol Chem. 279(44):46191-46203. PMID:15292237
  12. [ + ] Gerhard DS, et al. (2004) "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." Genome Res. 14(10B):2121-2127. PMID:15489334
  13. [ + ] Conner SD, et al. (2003) "Differential requirements for AP-2 in clathrin-mediated endocytosis." J Cell Biol. 162(5):773-779. PMID:12952931
  14. [ + ] Conner SD, et al. (2003) "AAK1-mediated micro2 phosphorylation is stimulated by assembled clathrin." Traffic. 4(12):885-890. PMID:14617351
  15. [ + ] Strausberg RL, et al. (2002) "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences." Proc Natl Acad Sci U S A. 99(26):16899-16903. PMID:12477932
  16. [ + ] Ricotta D, et al. (2002) "Phosphorylation of the AP2 mu subunit by AAK1 mediates high affinity binding to membrane protein sorting signals." J Cell Biol. 156(5):791-795. PMID:11877457
  17. [ + ] Manning G, et al. (2002) "The protein kinase complement of the human genome." Science. 298(5600):1912-1934. PMID:12471243
  18. [ + ] Conner SD, et al. (2002) "Identification of an adaptor-associated kinase, AAK1, as a regulator of clathrin-mediated endocytosis." J Cell Biol. 156(5):921-929. PMID:11877461
  19. [ + ] Kikuno R, et al. (1999) "Prediction of the coding sequences of unidentified human genes. XIV. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro." DNA Res. 6(3):197-205. PMID:10470851