EPHB1 | GeneID:2047 | Homo sapiens

Gene Summary

[ - ] NCBI Entrez Gene

Gene ID 2047 Official Symbol EPHB1
Locus N/A Gene Type protein-coding
Synonyms ELK; EPHT2; FLJ37986; Hek6; NET
Full Name EPH receptor B1
Description EPH receptor B1
Chromosome 3q21-q23
Also Known As eph tyrosine kinase 2; ephrin receptor EphB1; soluble EPHB1 variant 1
Summary Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The protein encoded by this gene is a receptor for ephrin-B family members. [provided by RefSeq]

Orthologs and Paralogs

[ - ] Homologs - NCBI's HomoloGene Group: 20936

ID Symbol Protein Species
GeneID:2047 EPHB1 NP_004432.1 Homo sapiens
GeneID:24338 Ephb1 XP_217250.1 Rattus norvegicus
GeneID:43803 Eph NP_524625.3 Drosophila melanogaster
GeneID:270190 Ephb1 NP_775623.2 Mus musculus
GeneID:396177 EPHB1 XP_422685.2 Gallus gallus
GeneID:460714 EPHB1 XP_001150963.1 Pan troglodytes
GeneID:485671 EPHB1 XP_542791.2 Canis lupus familiaris
GeneID:534731 EPHB1 XP_614602.3 Bos taurus
GeneID:1271751 AgaP_AGAP000489 XP_310604.2 Anopheles gambiae


[ - ] Monoclonal and Polyclonal Antibodies

No. Provider Product No. Description
1 abcam ab28314 Eph receptor B1 antibody (ab28314); Sheep polyclonal to Eph receptor B1
2 abcam ab5414 Eph receptor B1 antibody (ab5414); Rabbit polyclonal to Eph receptor B1
3 abcam ab72059 Eph receptor B1 antibody - C-terminal (ab72059); Rabbit polyclonal to Eph receptor B1 - C-terminal
4 abcam ab66326 Eph receptor B1 antibody [5F10A4] (ab66326); Mouse monoclonal [5F10A4] to Eph receptor B1
5 abcam ab61791 Eph receptor B1 + Eph receptor B2 (phospho Y594) antibody (ab61791); Rabbit polyclonal to Eph receptor B1 + Eph receptor B2 (phospho Y594)
6 abcam ab61765 Eph receptor B1 + Eph receptor B2 antibody (ab61765); Rabbit polyclonal to Eph receptor B1 + Eph receptor B2
7 abcam ab30562 Eph receptor B1 (phospho Y317) antibody (ab30562); Rabbit polyclonal to Eph receptor B1 (phospho Y317)
8 abcam ab30563 Eph receptor B1 (phospho Y331) antibody (ab30563); Rabbit polyclonal to Eph receptor B1 (phospho Y331)
9 abcam ab10406 Eph receptor B1 antibody (ab10406); Sheep polyclonal to Eph receptor B1
10 abcam ab10407 Eph receptor B1 antibody (ab10407); Sheep polyclonal to Eph receptor B1
11 abcam ab28312 Eph receptor B1 antibody (ab28312); Sheep polyclonal to Eph receptor B1
12 abgent AP7622d EphB1 Antibody; Purified Rabbit Polyclonal Antibody (Pab)
13 abgent AP7622a EphB1 Antibody (C-term); Purified Rabbit Polyclonal Antibody (Pab)
14 acris AP14295PU-N EphB1; antibody Ab
15 acris AP14294PU-N EphB1 (C-term); antibody Ab
16 scbt EPHB1 EPHB1 Antibody / EPHB1 Antibodies;
17 sigma E7517 Anti-EphB1 (ELK Receptor)-CY antibody produced in sheep ;
18 sigma E5892 Anti-EphB1 (ELK Receptor)-EXT antibody produced in sheep ;
19 sigma E6392 Anti-EphB1 (ELK Receptor)-SAM antibody produced in sheep ;

Exon, Intron and UTRs

Exon, Intron and UTRs of EPHB1 Gene Transcript Isoforms

CpG near TSS

CpG dinucleotides near Transcription Start Site of EPHB1 Gene

Gene Classification

[ - ] Gene Ontology

IDCategoryGO Term
GO:0005887 Component integral to plasma membrane
GO:0005886 Component plasma membrane
GO:0005524 Function ATP binding
GO:0005003 Function ephrin receptor activity
GO:0000166 Function nucleotide binding
GO:0005515 Function protein binding
GO:0004872 Function receptor activity
GO:0016740 Function transferase activity
GO:0007399 Process nervous system development
GO:0006468 Process protein amino acid phosphorylation
GO:0007165 Process signal transduction
GO:0007169 Process transmembrane receptor protein tyrosine kinase signaling pathway

RefSeq Isoforms

[ - ] RefSeq Annotation and UniProt Database

No. RefSeq RNA RefSeq Protein UniProt Equivalent
1 NM_004441  UCSC Browser NP_004432

Transcript Sequences

[ - ] Transcript Accession Number Cloud [ GenBank ]

Protein Sequences

[ - ] Protein Accession Number Cloud [ GenPept ]

Mutations and SNPs

[ - ] NCBI's dbSNP

[ - ] Somatic Mutations in Cancer - Sanger's COSMIC

The mutation data was obtained from the Sanger Institute Catalogue Of Somatic Mutations In Cancer web site, http://www.sanger.ac.uk/cosmic Bamford et al (2004) The COSMIC (Catalogue of Somatic Mutations in Cancer) database and website. Br J Cancer, 91,355-358.
Mutation (top 10)Total Observations
Primary Site / Histology (Top 10)Mutations (sites * observations)
stomach / carcinoma3
lung / carcinoma1
central nervous system / glioma1
ovary / carcinoma1


[ - ] Genes and Diseases - MIM at NCBI

Gene Interactions

[ - ] BioGRID Gene Product Interaction Database

Symbol Interaction Binary Experiment Source
ACP1 EPHB1 / ACP1 Affinity Capture-Western Stein E (1998)
ACP1 EPHB1 / ACP1 Reconstituted Complex Stein E (1998)
EFNA5 EFNA5 / EPHB1 Reconstituted Complex Kozlosky CJ (1997)
EFNB1 EFNB1 / EPHB1 Invitro Han DC (2002)
EFNB1 EFNB1 / EPHB1 Invivo Han DC (2002)
GRB10 EPHB1 / GRB10 Two-hybrid Stein E (1996)
GRB2 EPHB1 / GRB2 Two-hybrid Stein E (1996)
NCK1 NCK1 / EPHB1 Two-hybrid Stein E (1998)

Transcript Cluster

[ - ] NCBI's UniGene

Selected Publications

[ - ] Gene-related publications indexed at PubMed

  1. [ + ] Fasen K, et al. (2008) "Ligand binding induces Cbl-dependent EphB1 receptor degradation through the lysosomal pathway." Traffic. 9(2):251-266. PMID:18034775
  2. [ + ] Li Y, et al. (2008) "Neither replication nor simulation supports a role for the axon guidance pathway in the genetics of Parkinson's disease." PLoS ONE. 3(7):e2707. PMID:18628988
  3. [ + ] Ng D, et al. (2008) "Replication of a genome-wide case-control study of esophageal squamous cell carcinoma." Int J Cancer. 123(7):1610-1615. PMID:18649358
  4. [ + ] Sheng Z, et al. (2008) "EphB1 is underexpressed in poorly differentiated colorectal cancers." Pathobiology. 75(5):274-280. PMID:18931529
  5. [ + ] Jin P, et al. (2008) "Novel splice variants derived from the receptor tyrosine kinase superfamily are potential therapeutics for rheumatoid arthritis." Arthritis Res Ther. 10(4):R73. PMID:18593464
  6. [ + ] Wang JD, et al. (2007) "Loss of expression of EphB1 protein in gastric carcinoma associated with invasion and metastasis." Oncology. 73(3-4):238-245. PMID:18424888
  7. [ + ] Chumley MJ, et al. (2007) "EphB receptors regulate stem/progenitor cell proliferation, migration, and polarity during hippocampal neurogenesis." J Neurosci. 27(49):13481-13490. PMID:18057206
  8. [ + ] Zhang Y, et al. (2005) "Time-resolved mass spectrometry of tyrosine phosphorylation sites in the epidermal growth factor receptor signaling network reveals dynamic modules." Mol Cell Proteomics. 4(9):1240-1250. PMID:15951569
  9. [ + ] Koolpe M, et al. (2005) "EphB receptor-binding peptides identified by phage display enable design of an antagonist with ephrin-like affinity." J Biol Chem. 280(17):17301-17311. PMID:15722342
  10. [ + ] Zhang Z, et al. (2004) "Signal peptide prediction based on analysis of experimentally verified cleavage sites." Protein Sci. 13(10):2819-2824. PMID:15340161
  11. [ + ] Vindis C, et al. (2004) "EphB1-mediated cell migration requires the phosphorylation of paxillin at Tyr-31/Tyr-118." J Biol Chem. 279(27):27965-27970. PMID:15107421
  12. [ + ] Ota T, et al. (2004) "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat Genet. 36(1):40-45. PMID:14702039
  13. [ + ] Prevost N, et al. (2004) "Signaling by ephrinB1 and Eph kinases in platelets promotes Rap1 activation, platelet adhesion, and aggregation via effector pathways that do not require phosphorylation of ephrinB1." Blood. 103(4):1348-1355. PMID:14576067
  14. [ + ] Vindis C, et al. (2003) "EphB1 recruits c-Src and p52Shc to activate MAPK/ERK and promote chemotaxis." J Cell Biol. 162(4):661-671. PMID:12925710
  15. [ + ] Strausberg RL, et al. (2002) "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences." Proc Natl Acad Sci U S A. 99(26):16899-16903. PMID:12477932
  16. [ + ] Han DC, et al. (2002) "EphB1 associates with Grb7 and regulates cell migration." J Biol Chem. 277(47):45655-45661. PMID:12223469
  17. [ + ] Freywald A, et al. (2002) "The kinase-null EphB6 receptor undergoes transphosphorylation in a complex with EphB1." J Biol Chem. 277(6):3823-3828. PMID:11713248
  18. [ + ] Huynh-Do U, et al. (2002) "Ephrin-B1 transduces signals to activate integrin-mediated migration, attachment and angiogenesis." J Cell Sci. 115(Pt 15):3073-3081. PMID:12118063
  19. [ + ] Prevost N, et al. (2002) "Interactions between Eph kinases and ephrins provide a mechanism to support platelet aggregation once cell-to-cell contact has occurred." Proc Natl Acad Sci U S A. 99(14):9219-9224. PMID:12084815
  20. [ + ] Wilkinson DG, et al. (2001) "Multiple roles of EPH receptors and ephrins in neural development." Nat Rev Neurosci. 2(3):155-164. PMID:11256076
  21. [ + ] Comings DE, et al. (2000) "A multivariate analysis of 59 candidate genes in personality traits: the temperament and character inventory." Clin Genet. 58(5):375-385. PMID:11140838
  22. [ + ] Becker E, et al. (2000) "Nck-interacting Ste20 kinase couples Eph receptors to c-Jun N-terminal kinase and integrin activation." Mol Cell Biol. 20(5):1537-1545. PMID:10669731
  23. [ + ] Wilkinson DG, et al. (2000) "Eph receptors and ephrins: regulators of guidance and assembly." Int Rev Cytol. 196():177-244. PMID:10730216
  24. [ + ] Xu Q, et al. (2000) "Roles of Eph receptors and ephrins in segmental patterning." Philos Trans R Soc Lond B Biol Sci. 355(1399):993-1002. PMID:11128993
  25. [ + ] Holder N, et al. (1999) "Eph receptors and ephrins: effectors of morphogenesis." Development. 126(10):2033-2044. PMID:10207129
  26. [ + ] Yue Y, et al. (1999) "Specification of distinct dopaminergic neural pathways: roles of the Eph family receptor EphB1 and ligand ephrin-B2." J Neurosci. 19(6):2090-2101. PMID:10066262
  27. [ + ] Zhou R, et al. (1998) "The Eph family receptors and ligands." Pharmacol Ther. 77(3):151-181. PMID:9576626
  28. [ + ] Flanagan JG, et al. (1998) "The ephrins and Eph receptors in neural development." Annu Rev Neurosci. 21():309-345. PMID:9530499
  29. [ + ] Stein E, et al. (1998) "Eph receptors discriminate specific ligand oligomers to determine alternative signaling complexes, attachment, and assembly responses." Genes Dev. 12(5):667-678. PMID:9499402
  30. [ + ] Abrahamson DR, et al. (1998) "Origins and formation of microvasculature in the developing kidney." Kidney Int Suppl. 67():S7-11. PMID:9736245
  31. [ + ] Stein E, et al. (1998) "Nck recruitment to Eph receptor, EphB1/ELK, couples ligand activation to c-Jun kinase." J Biol Chem. 273(3):1303-1308. PMID:9430661
  32. [ + ] Kozlosky CJ, et al. (1997) "LERK-7: a ligand of the Eph-related kinases is developmentally regulated in the brain." Cytokine. 9(8):540-549. PMID:9245480
  33. [ + ] Stein E, et al. (1996) "Ligand activation of ELK receptor tyrosine kinase promotes its association with Grb10 and Grb2 in vascular endothelial cells." J Biol Chem. 271(38):23588-23593. PMID:8798570
  34. [ + ] Bonaldo MF, et al. (1996) "Normalization and subtraction: two approaches to facilitate gene discovery." Genome Res. 6(9):791-806. PMID:8889548
  35. [ + ] Gale NW, et al. (1996) "Eph receptors and ligands comprise two major specificity subclasses and are reciprocally compartmentalized during embryogenesis." Neuron. 17(1):9-19. PMID:8755474
  36. [ + ] Tang XX, et al. (1995) "cDNA cloning, molecular characterization, and chromosomal localization of NET(EPHT2), a human EPH-related receptor protein-tyrosine kinase gene preferentially expressed in brain." Genomics. 29(2):426-437. PMID:8666391
  37. [ + ] Cerretti DP, et al. (1995) "Isolation of LERK-5: a ligand of the eph-related receptor tyrosine kinases." Mol Immunol. 32(16):1197-1205. PMID:8559144
  38. [ + ] Davis S, et al. (1994) "Ligands for EPH-related receptor tyrosine kinases that require membrane attachment or clustering for activity." Science. 266(5186):816-819. PMID:7973638
  39. [ + ] Beckmann MP, et al. (1994) "Molecular characterization of a family of ligands for eph-related tyrosine kinase receptors." EMBO J. 13(16):3757-3762. PMID:8070404
  40. [ + ] Larose L, et al. (1993) "Identification of residues in the beta platelet-derived growth factor receptor that confer specificity for binding to phospholipase C-gamma 1." Oncogene. 8(9):2493-2499. PMID:7689724