PIGK | GeneID:10026 | Homo sapiens

Gene Summary

[ - ] NCBI Entrez Gene

Gene ID 10026 Official Symbol PIGK
Locus RP4-564M11.1 Gene Type protein-coding
Synonyms GPI8; MGC22559
Full Name phosphatidylinositol glycan anchor biosynthesis, class K
Description phosphatidylinositol glycan anchor biosynthesis, class K
Chromosome 1p31.1
Also Known As GPI transamidase subunit; OTTHUMP00000011255; phosphatidylinositol glycan, class K
Summary This gene encodes a member of the cysteine protease family C13 that is involved in glycosylphosphatidylinositol (GPI)-anchor biosynthesis. The GPI-anchor is a glycolipid found on many blood cells and serves to anchor proteins to the cell surface. This protein is a member of the multisubunit enzyme, GPI transamidase and is thought to be its enzymatic component. GPI transamidase mediates GPI anchoring in the endoplasmic reticulum, by catalyzing the transfer of fully assembled GPI units to proteins. [provided by RefSeq]

Orthologs and Paralogs

[ - ] Homologs - NCBI's HomoloGene Group: 4002

ID Symbol Protein Species
GeneID:10026 PIGK NP_005473.1 Homo sapiens
GeneID:31163 CG4406 NP_569968.2 Drosophila melanogaster
GeneID:188131 T05E11.6 NP_502076.1 Caenorhabditis elegans
GeneID:295543 Pigk NP_001011953.1 Rattus norvegicus
GeneID:329777 Pigk NP_079938.1 Mus musculus
GeneID:415239 pigk NP_001002149.1 Danio rerio
GeneID:424556 PIGK NP_001026449.1 Gallus gallus
GeneID:456962 PIGK XP_001168438.1 Pan troglodytes
GeneID:479985 PIGK XP_853775.1 Canis lupus familiaris
GeneID:508700 PIGK XP_001250165.1 Bos taurus
GeneID:837397 AT1G08750 NP_563825.1 Arabidopsis thaliana
GeneID:851931 GPI8 NP_010618.1 Saccharomyces cerevisiae
GeneID:1274450 AgaP_AGAP004301 XP_313575.2 Anopheles gambiae
GeneID:2539272 gpi8 NP_588198.1 Schizosaccharomyces pombe
GeneID:2682057 MGG_10490 XP_366271.1 Magnaporthe grisea
GeneID:2709454 NCU07259.1 XP_327545.1 Neurospora crassa
GeneID:2894186 KLLA0E17105g XP_454718.1 Kluyveromyces lactis
GeneID:4328747 Os02g0219400 NP_001046312.1 Oryza sativa
GeneID:4620562 AGOS_ADR299W NP_984396.1 Eremothecium gossypii

Antibodies

[ - ] Monoclonal and Polyclonal Antibodies

No. Provider Product No. Description
1 abcam ab38443 PIGK antibody (ab38443); Rabbit polyclonal to PIGK
2 abcam ab38445 PIGK antibody (ab38445); Rabbit polyclonal to PIGK
3 abgent AP2462b GPI8 Antibody (C-term); Purified Rabbit Polyclonal Antibody (Pab)
4 abgent AP2462a GPI8 Antibody (N-term); Purified Rabbit Polyclonal Antibody (Pab)
5 acris AP12208PU-N GPI8 (N-term); antibody Ab
6 acris AP12209PU-N GPI8 (C-term); antibody Ab
7 scbt PIGK PIGK Antibody / PIGK Antibodies;

Exon, Intron and UTRs

Exon, Intron and UTRs of PIGK Gene Transcript Isoforms

CpG near TSS

CpG dinucleotides near Transcription Start Site of PIGK Gene

Gene Classification

[ - ] Gene Ontology

IDCategoryGO Term
GO:0005783 Component endoplasmic reticulum
GO:0005789 Component endoplasmic reticulum membrane
GO:0042765 Component GPI-anchor transamidase complex
GO:0016021 Component integral to membrane
GO:0016020 Component membrane
GO:0004197 Function cysteine-type endopeptidase activity
GO:0034235 Function GPI anchor binding
GO:0003923 Function GPI-anchor transamidase activity
GO:0008233 Function peptidase activity
GO:0005515 Function protein binding
GO:0016255 Process attachment of GPI anchor to protein
GO:0006467 Process protein thiol-disulfide exchange
GO:0006508 Process proteolysis

RefSeq Isoforms

[ - ] RefSeq Annotation and UniProt Database

No. RefSeq RNA RefSeq Protein UniProt Equivalent
1 NM_005482  UCSC Browser NP_005473 B2R7K3   Q92643  

Transcript Sequences

[ - ] Transcript Accession Number Cloud [ GenBank ]

Protein Sequences

[ - ] Protein Accession Number Cloud [ GenPept ]

Mutations and SNPs

[ - ] NCBI's dbSNP

Phenotypes

[ - ] Genes and Diseases - MIM at NCBI

Chemicals and Drugs

[ - ] Comparative Toxicogenomics Database from MDI Biological Lab

Curated [chemical–gene interactions|chemical–disease|gene–disease] data were retrieved from the Comparative Toxicogenomics Database (CTD), Mount Desert Island Biological Laboratory, Salisbury Cove, Maine. World Wide Web (URL: http://ctd.mdibl.org/). [Jan. 2009].
Chemical and Interaction
Acetaminophen
  • Acetaminophen affects the expression of PIGK mRNA
17562736
Carbon Tetrachloride
  • Carbon Tetrachloride affects the expression of PIGK mRNA
17484886
sodium arsenite
  • sodium arsenite results in increased expression of PIGK mRNA
16014739

Gene and Diseases

[ - ] Gene and Diseases [Data source: CTD]

Curated [chemical–gene interactions|chemical–disease|gene–disease] data were retrieved from the Comparative Toxicogenomics Database (CTD), Mount Desert Island Biological Laboratory, Salisbury Cove, Maine. World Wide Web (URL: http://ctd.mdibl.org/). [Jan. 2009].
Disease Name Relationship PubMed
Adrenal Gland Neoplasms inferred via sodium arsenite 15276417
Adrenocortical Adenoma inferred via sodium arsenite 16712894
Carcinoma, Hepatocellular inferred via sodium arsenite 15276417, 16507464
Carcinoma, Squamous Cell inferred via sodium arsenite 18572023
Genital Neoplasms, Female inferred via sodium arsenite 16452187
Hodgkin Disease inferred via sodium arsenite 12676792
Liver Neoplasms inferred via sodium arsenite 15276417, 16712894
Lung Neoplasms inferred via sodium arsenite 15276417, 16712894, 17077188
Melanoma inferred via sodium arsenite 16487513
Neoplasms inferred via sodium arsenite 11559025
Neural Tube Defects inferred via sodium arsenite 12854658
Ovarian Neoplasms inferred via sodium arsenite 15276417
Prostatic Neoplasms inferred via sodium arsenite 16039940
Skin Neoplasms inferred via sodium arsenite 18572023
Spinal Dysraphism inferred via sodium arsenite 12854658
Urinary Bladder Neoplasms inferred via sodium arsenite 11723127, 16452187, 16712894
Uterine Cervical Neoplasms inferred via sodium arsenite 11813266
Vascular Diseases inferred via sodium arsenite 17056641
Carbon Tetrachloride Poisoning inferred via Carbon Tetrachloride 16192424, 15673190, 16050911, 16011737, 15700767, 16124888, 16227642, 10355542, 16097048
Fatty Liver inferred via Carbon Tetrachloride 16045604, 16239168, 12795759, 12631006, 61145, 17595544, 15959796
Hepatitis, Toxic inferred via Carbon Tetrachloride 17522070, 16177239, 15027814, 15968718, 16227642, 15998439, 11566570
Hyperbilirubinemia inferred via Carbon Tetrachloride 16899240
Liver Cirrhosis inferred via Carbon Tetrachloride 17174718, 16943688, 16221502, 17334410, 16239168
Liver Cirrhosis, Experimental inferred via Carbon Tetrachloride 16192424, 17557913, 15052691, 12632512, 17766677, 18418968, 12666154, 16638106, 18395095, 18156304, 17976157, 17565644, 17869086, 17708605, 12667390, 14748882, 13678700, 15818738, 17631135, 16097048, 15673190, 12649538, 17721639, 18277467, 18205269, 14716496, 15730626, 12632514, 18376398, 12389079, 18187930, 18210741, 16015684, 12741479, 14724832, 18472094, 15931870, 17698563, 15893842, 12958196, 17640975, 18412020, 17714472, 14512876, 12609069, 18166357, 17922224, 18420326, 15876570, 12445421, 12445418, 15959796, 12898905, 18317297, 17761835, 14620537, 18472332, 14716833, 16136751, 17481882, 17900296, 15123356, 18339082, 18429990, 12546737, 18006644, 18481824, 15057751, 12586293, 18054572, 10355542, 16011737, 18251166, 17823541, 17944888, 18395914, 18279442, 16027843, 15996030, 16033810, 17805973, 16116963, 15925388, 16248980, 17525996
Liver Diseases inferred via Carbon Tetrachloride 16246199, 16964402, 17285989, 15830285, 15720792
Liver Failure inferred via Carbon Tetrachloride 15123358
Liver Failure, Acute inferred via Carbon Tetrachloride 14706259, 16899240
Liver Neoplasms, Experimental inferred via Carbon Tetrachloride 15583823
Hepatitis, Toxic inferred via Acetaminophen 2444490, 16081117, 17522070, 14986274, 16227642, 15968718, 16177239, 17562736
Hyperalgesia inferred via Acetaminophen 16870215
Liver Failure, Acute inferred via Acetaminophen 16871587, 17185352
Pain inferred via Acetaminophen 16870215

Gene Interactions

[ - ] BioGRID Gene Product Interaction Database

Symbol Interaction Binary Experiment Source
ALPP PIGK / ALPP Reconstituted Complex Spurway TD (2001)
CANX PIGK / CANX Affinity Capture-MS Ohishi K (2003)
GPAA1 PIGK / GPAA1 Affinity Capture-Western Ohishi K (2000)
GPAA1 GPAA1 / PIGK Affinity Capture-Western Vainauskas S (2002)
GPAA1 PIGK / GPAA1 Co-fractionation Vainauskas S (2002)
PIGT PIGK / PIGT Affinity Capture-Western Ohishi K (2003)
PIGT PIGK / PIGT Reconstituted Complex Ohishi K (2003)
ULBP2 PIGK / ULBP2 Affinity Capture-MS Ohishi K (2003)

Transcript Cluster

[ - ] NCBI's UniGene

Selected Publications

[ - ] Gene-related publications indexed at PubMed

  1. [ + ] Kimura K, et al. (2006) "Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes." Genome Res. 16(1):55-65. PMID:16344560
  2. [ + ] Gregory SG, et al. (2006) "The DNA sequence and biological annotation of human chromosome 1." Nature. 441(7091):315-321. PMID:16710414
  3. [ + ] Gerhard DS, et al. (2004) "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." Genome Res. 14(10B):2121-2127. PMID:15489334
  4. [ + ] Ota T, et al. (2004) "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat Genet. 36(1):40-45. PMID:14702039
  5. [ + ] Chen R, et al. (2003) "Proprotein interaction with the GPI transamidase." J Cell Biochem. 88(5):1025-1037. PMID:12616539
  6. [ + ] Hong Y, et al. (2003) "Human PIG-U and yeast Cdc91p are the fifth subunit of GPI transamidase that attaches GPI-anchors to proteins." Mol Biol Cell. 14(5):1780-1789. PMID:12802054
  7. [ + ] Ohishi K, et al. (2003) "Two subunits of glycosylphosphatidylinositol transamidase, GPI8 and PIG-T, form a functionally important intermolecular disulfide bridge." J Biol Chem. 278(16):13959-13967. PMID:12582175
  8. [ + ] Strausberg RL, et al. (2002) "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences." Proc Natl Acad Sci U S A. 99(26):16899-16903. PMID:12477932
  9. [ + ] Vainauskas S, et al. (2002) "Structural requirements for the recruitment of Gaa1 into a functional glycosylphosphatidylinositol transamidase complex." J Biol Chem. 277(34):30535-30542. PMID:12052837
  10. [ + ] Spurway TD, et al. (2001) "Early events in glycosylphosphatidylinositol anchor addition. substrate proteins associate with the transamidase subunit gpi8p." J Biol Chem. 276(19):15975-15982. PMID:11278620
  11. [ + ] Ohishi K, et al. (2001) "PIG-S and PIG-T, essential for GPI anchor attachment to proteins, form a complex with GAA1 and GPI8." EMBO J. 20(15):4088-4098. PMID:11483512
  12. [ + ] Ohishi K, et al. (2000) "Gaa1p and gpi8p are components of a glycosylphosphatidylinositol (GPI) transamidase that mediates attachment of GPI to proteins." Mol Biol Cell. 11(5):1523-1533. PMID:10793132
  13. [ + ] Meyer U, et al. (2000) "Active site determination of Gpi8p, a caspase-related enzyme required for glycosylphosphatidylinositol anchor addition to proteins." Biochemistry. 39(12):3461-3471. PMID:10727241
  14. [ + ] Yu J, et al. (1997) "The affected gene underlying the class K glycosylphosphatidylinositol (GPI) surface protein defect codes for the GPI transamidase." Proc Natl Acad Sci U S A. 94(23):12580-12585. PMID:9356492
  15. [ + ] Benghezal M, et al. (1996) "Yeast Gpi8p is essential for GPI anchor attachment onto proteins." EMBO J. 15(23):6575-6583. PMID:8978684
  16. [ + ] Ploug M, et al. (1991) "Cellular receptor for urokinase plasminogen activator. Carboxyl-terminal processing and membrane anchoring by glycosyl-phosphatidylinositol." J Biol Chem. 266(3):1926-1933. PMID:1846368