ABCB5 (Internal) | Homo sapiens | acris

Specification of AP09395PU-N

Vendor Acris Antibodies GmbH Gene ID 340273
Catalog No. AP09395PU-N Gene Symbol ABCB5
Species Homo sapiens
Title ABCB5 (Internal)
Product Type antibody Ab
Host Rabbit
Isotype IgG
Validation

Western blot using affinity purified anti-ABCB5 antibody shows detection of ABCB5 beta in 12.5 μg of transfected-Hi5 whole cell lysate. No reaction was seen when antibody was pre-incubated with the immunizing peptide (data not shown). A 3-8% Tris-acetate gel was used for separation. The arrowhead corresponds to 117 kDa ABCB5. The membrane was probed with the primary antibody at a 1:10,000 dilution in 5% milk in TBST at 4° C, overnight.

Presentation Aff - Purified
Applications E, WB
Reactivity Hu, Mky
UniProt Q2M3G0
Synonyms ATP-binding cassette sub-family B member 5
State Liquid purified Ig
Purification Affinity chromatography
Buffer System 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01% (w/v) Sodium Azide
Storage Store the antibody at -20°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch. Storage Conditions for Trial Size: This vial contains a relatively low volume of reagent (25 μl). To minimize loss of volume dilute 1:10 by adding 225 μl of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Shelf life: 3 month from despatch.