Phospho-Gab1-Y627 Antibody | Homo sapiens | abgent

Specification of AP3109a

Vendor Abgent Inc Gene ID 2549
Catalog No. AP3109a Gene Symbol GAB1
Species Homo sapiens
Title Phospho-Gab1-Y627 Antibody
Alternative Title Purified Rabbit Polyclonal Antibody (Pab)
Specificity This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y627 of human GAB1.
Applications IHC, DB, E
Cross Reaction H
Clonality Polyclonal
Isotype Rabbit Ig
Western Blot

Dot blot analysis of anti-Phospho-Gab1-Y627 Antibody (Cat.#AP3109a) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antobodies working concentration was 0.5ug per ml.

IHC

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Precaution This product is for research use only. Not for use in diagnostic or therapeutic procedures.
Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.