| Vendor |
Abgent Inc |
Gene ID |
25 |
| Catalog No. |
AP3013a |
Gene Symbol |
ABL1 |
| Species |
Homo sapiens |
| Title |
Phospho-ABL1-Y245 Antibody |
| Alternative Title |
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab) |
| Specificity |
This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y245 of human ABL1. |
| Applications |
WB, IHC, E |
| Cross Reaction |
H |
| Clonality |
Polyclonal |
| Isotype |
Rabbit Ig |
| Western Blot |  Western blot analysis of anti-ABL Pab (Cat. #AP3013a) in mouse colon tissue lysate (35ug/lane). ABL(arrow) was detected using the purified Pab (1:30 dilution).
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| IHC |  Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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| Format |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
| Precaution |
This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Storage |
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C. |