ACO2 Antibody (Center) | Homo sapiens | abgent

Specification of AP1936c

Vendor Abgent Inc Gene ID 50
Catalog No. AP1936c Gene Symbol ACO2
Species Homo sapiens
Title ACO2 Antibody (Center)
Alternative Title Purified Rabbit Polyclonal Antibody (Pab)
Specificity This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the central region of human Aconitase.
Applications WB, E
Cross Reaction H, M, R
Clonality Polyclonal
Isotype Rabbit Ig
Western Blot

LEFT: Western blot analysis of anti-ACO2 Pab (Cat. #AP1936c) in mouse heart(left) and 293 (right) tissue lysates (35ug/lane). ACO2(arrow) was detected using the purified Pab (1:60 dilution). RIGHT: Perfused isolated rat heart whole tissue lysate was lysed with either A) 50 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, 1% NP-40, 0.1% SDS, 0.5% Na-deoxycholate, 1 mM Na3VO4, 20 mM NaF, 1 mM PMSF, 5 v/v % protease inhibitor cocktail or B) T-PER Tissue Protein Extraction Reagent [# 785101; Pierce], containing 1mM Na3VO4, 20 mM NaF, 5 v/v % protease inhibitor cocktail (Sigma); PVDF membrane was incubated in primary Ab [rabbit polyclonal antibody against ACO2 (Center) (Cat# AP1936c). Solution: 1:1000 diluted in 5% NFM TBS-T 0,05 for overnight (15 hrs) at 4 ÂșC. Data courtesy of Boglarka Laczy M.D., Division of Cardiovascular Disease, Dept. of Medicine, University of Alabama at Birmingham.

Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Precaution This product is for research use only. Not for use in diagnostic or therapeutic procedures.
Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.